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2.4 High-performance liquid chromatography-tandem mass spectrometry analysis of floret ergothioneine concentrations EGT was extracted from the florets and analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) as described by v / v ) HPLC-grade methanol, and shaken on a vortex platform for 10 min

Acknowledgements The authors are supported in part by research funds from the Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, OH, USA

This procedure requires several sessions to clear out the heavy metals

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It typically includes ingredients like Methionine, Inositol, and Choline (often called 'MIC'), alongside other supportive elements like B vitamins