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2.3 Biochemical analysis of the serum and liver of the mice Serum samples were thawed on ice and analyzed for AST, ALT, TG, and TC levels using commercially available assay kits following the manufacturers instructions

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11,12 Despite the consistent results showing benefit of NADs in reducing HE and its related mortality, these RCTs did not assess the confounding effect of factors precipitating HE since strategies directed at management of precipitating factors may improve HE with or without NADs