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CB6F1 female mice were obtained from the National Institute on Aging aged rodent colony at 15 months of age

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escalating protocols (e.g., Retatrutide, Semaglutide) will increase costs over time For maintenance phases (e.g., Melanotan) or as-needed use (e.g., PT-141), conservative estimates are applied Costs are rounded to two decimal places and represent active use only (excluding off-periods in cycles) Ready to Optimize Your Health with Peptide Therapy

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Sequencing was carried out by Sanger methodology on ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and 100% concordance with restriction fragment length polymorphism assays was shown providing confidence in using the full dataset in subsequent analysis