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Most frequently, FCS, which, in addition to the translational diffusion time of the molecules through the small detection volume provides information on the average number of fluorescent molecules in the detection volume, is utilized in detection and identification of single molecules in solution (Eigen and Rigler, 1994

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And although it was coined nearly 20 years ago, it's still relevant, perhaps even more so, in the era of GLP-1s: Eat food