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Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min, and the clarified cell lysate was directly used for biocatalytic reactions

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Special thanks The authors wish to express gratitude to the Al-Sadir Teaching Hospital staff for their cooperation with this study and to all research volunteers

Among these, aOoC systems represent a significant advancement, integrating patient-derived target and immune cells to create personalized microenvironments that profoundly enhance disease modeling, drug screening, and regenerative medicine applications