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Nonaka H, Ohue-Kitano R, Masujima Y, Igarashi M, Kimura I

Martin-Biggers et al

Cells were maintained in an incubator at 37 C and 5% CO 2 incubator and seeded onto 6-well cell culture plates before transfection

Fmoc deprotection was achieved with 20% piperidine in DMF (twice for 2 min, room temperature, 300 rpm shaking) and peptide couplings were performed as double or triple couplings (twice for 5 min, 75 C, 300 rpm shaking, except for Arg and His, for which twice for 5 min, 50 C, 300 rpm shaking) consisting of AA/HCTU/DIPEA (ratio, 1:1.25:2.5) in fivefold excess compared to the resin

Gs/Gq signaling switch in cells defines incretin effectiveness in diabetes