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Check for drug interactions - Some prescription medicines, like metformin, can deplete B12 over time

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This aggressive course is likely attributable to microscopic residual tumor following an R2-level subtotal resection, which may have seeded early micrometastases

Briefly, C18 columns were activated by washing once with 400 L of acetonitrile (Fisher Scientific) and twice with 400 L of 0.1% formic acid (Thermo Scientific) in water solution, LCMS grade (Thermo Scientific)