Protein concentrations were determined using the Bio-Rad protein assay (Bio-Rad, CA, USA) based on the Bradford method ( ASSAYS OF GGT ENZYME ACTIVITIES Assays of GGT enzyme activities were performed by analyzing the amount of deglutamylated compounds produced from -glutamylated compounds by yeast crude proteins in 6 h at 37C
Such techniques may include ultrafiltration using membranes, ion exchange methods, and membrane methods, for example
Ting Chen National Institute of Biological Sciences, Beijing, China Contribution TC, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents For correspondence [email protected] Competing interests The authors declare that no competing interests exist
The timeline will depend on safety data, final trial outcomes, and regulatory review
Along with the regeneration of AsA from MDHA, chloroplastic MDHAR also brings about the photo-reduction of dioxygen to O 2 in absence of MDHA (Miyake et al., 1998
