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Next, the blood levels of LPO were analyzed with enzyme-linked immunosorbent assay (ELISA) to determine whether the Si-based agent suppresses oxidative metabolites in the blood

Supernatants were subsequently acidified with 0.5% trifluoroacetic acid (TFA), followed by desalting using reversed-phase spin tips (Glygen Corp) and drying using vacuum centrifugation

Z.et al (1995)

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