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12345) was employed, which uses a grid of infrared beams to detect and record the animals movements, providing precise measurements of wakefulness time

Gene expression was quantified using SYBR green real-time PCR master mix (TOYOBO, Japan) and amplified under the following conditions: 2 min at 95C, 20 s at 95C, and 20 s at 58C, for 40 cycles

Crit Rev Biochem Mol Biol 48(2):173181 Rigobello MP, Bindoli A (2010) Mitochondrial thioredoxin reductase: purification, inhibitor studies, and role in cell signaling

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This effect arises from deviation from ideality of the activity coefficients of the solute species