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Real-time PCR was performed using an SYBR Green I technology and MxPro- Mx3000P QPCR machine (Stratagene, CA, USA), and a master mix was prepared with Smart Quant Green Master Mix with dUTP & ROX Kit (Protech, Taipei, Taiwan)
Comment: Many commenters reiterated their longstanding recommendation that CMS discontinue the ASC weight scalar, stating that the secondary rescaling process applied to maintain budget neutrality has created an inappropriate and growing payment disparity between ASCs and HOPDs, with ASC reimbursement rates now averaging 50 percent less than HOPD rates for the same procedures despite no evidence of increased differences in capital and operating costs between settings
doi: 10.1126/sciimmunol.aaj2195 100 LeeJHKimBJinWJKimHHHaHLeeZH
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