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This was followed by a blocking step with 10% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize non-specific binding

These may include: Nausea and stomach upset Diarrhea Constipation Injection site redness or swelling Itching Headache Dizziness Blood clots Swelling In rare cases, some people may experience an allergic reaction to vitamin B12, which can cause symptoms such as hives, difficulty breathing, and swelling of the face, lips, tongue, or throat

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Buvelot, H., Jaquet, V

10.1039/c9bm01284b Summary Keywords cell transfection, glutathione S-transferase, glutathione, GPI, cell sorting Citation Ma S, Yang L, Zuo Q and Huang Q (2022) GPI-anchored glutathione S-transferase as marker allows affinity sorting of transfection-positive cells