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Although statistically significant, these effects remain smaller than those achieved with retinoids or growth factor formulations

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There is also limited research on individualized dietary strategies tailored to specific PCOS phenotypes (e.g., lean vs

At 48 h post-transduction, cells were fixed in 2% paraformaldehyde (PFA) for 10 min and, after blocking with 3% BSA in PBS, were incubated with 10 mg/ml anti-H105A antibody (clone number AbD57564pao) diluted in 1% BSA and 0.1% Tween 20 at 4 C for 16 h, followed by incubation with secondary antibody, anti-FLAG M2 antibody (Merck, catalogue number F1804) diluted 1:100 in 1% BSA and 0.1% Tween 20 at 25 C for 2 h and Alexa Fluor 568 anti-mouse as tertiary antibody (Thermo Fisher Scientific, catalogue number A-11004) diluted 1:1000 in 0.1% BSA at 25 C for 1 h