To ensure consistent peptide concentrations across samples, the digested peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay (#23275, Thermo Fisher Scientific), a modified BCA assay
Oral supplements have limited bioavailability due to digestion a large portion is broken down before reaching the cells
In particular, these effects all appeared in conjunction with BPC 157 application
This isn't like stopping exogenous testosterone, where you risk hypogonadism without PCT
Sci Rep 6:31899 Chin Y-T, Wang L-M, Hsieh M-T, Shih Y-J, Nana AW, Changou CA, Yang Y-CS, Chiu H-C, Fu E et al (2017) Leptin OB3 peptide suppresses leptin-induced signaling and progression in ovarian cancer cells