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In addition to MTT assays, we also performed cell counting with a Countess Automated Cell Counter (Applied Biosystems) in experiments where we treated primary melanoma cell lines WM115 and WM1862 with the EPAC inhibitor ESI-09 and transfected with EPAC siRNA

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Small human studies have reported increases in glutathione measurements and selected oxidative-stress or immune markers following use of a tested liposomal formulation

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The anti-inflammatory effects of omega-3s help calm overactive sebaceous glands and reduce the inflammatory response that worsens acne