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J.EvensenK

The absorbance of the supernatant was measured spectrophotometrically at 532 nm

Free-energy calculations The interaction free energies ( G ) of P i and ND1L-p97 (wild type in states A and B as well as the mutant R359K) were calculated using the MMPBSA single-trajectory method as implemented in the MMPBSA.py 33 script, which is part of the AMBER18 package 53