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The PCNA immunohistochemical score was computed using the percentage and staining intensity of brown positive nuclear cells within the field of vision as follows: 0 (no positive cells), 1 (110% positive cells), 2 (1150% positive cells), 3 (5180% positive cells), and 4 (81100% positive cells)

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While the next SM Breeding Protocol, scheduled to be released in 2012 by the British Veterinary Association and the Kennel Club (BVA/KC), still is a work-in-progress, a preliminary draft of it (see below) would permit the breeding of cavaliers as young as 12 months old

This study lacked a placebo control, so results should be interpreted cautiously