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Rats were pretreated with icv scopolamine (70 nmol in 2 aCSF) 20 min prior to training followed by the icv infusion of dihexa (0.1 or 1 nmol in 2 aCSF) 5 min prior to daily training

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During the public comment period held during measurement development, commenters strongly agreed with this approach and appreciated its alignment with other CMS reporting periods

A pivotal 2007 study published in the Journal of Physiology and Pharmacology examined rats with experimentally induced esophagitis and sphincter failure