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(2006) Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults, J Clin Endocrinol Metab, 91(3), pp

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For the induction of recombinant proteins, the yeast cells grown in SD minimal medium without uracil at 28C for 1 days were transferred to 10 volumes of uracil-less SD medium containing 2% (w/v) galactose instead of glucose to activate the GAL1 promoter on pYES2, and cultured at 28C for 1 days

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They were spiked into a stable background of tryptic yeast phosphoproteome sample in different concentrations (1, 10, 100, 1000) and measured in triplicates using DDA and DIA (Fig