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Structural deficits include EC degeneration, detachment of astrocytic endfeet, and pericyte degeneration that cause instability of the vascular architecture, as well as reduced expression of TJ proteins claudin-5 and occludin (Winkler et al., 2011

One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)