Most of our knowledge to date regarding the evolution of neurochemical changes post injury stems from studies which used ex vivo 1 H-NMR technique or from pre-clinical studies performed in adult animals [17, 62, 63]
For example, metabolic disorders like type 2 diabetes involve a complex interplay between pancreatic, hepatic, adipose, and immune cells (187), while cancer progression and drug resistance are increasingly linked to interactions between tumor cells, stromal fibroblasts, immune infiltrates, and endothelial components (188, 189)
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In addition to this, completing all three stages of a clinical trial takes time an estimated 10-15 years to be fully cleared for use (3)
mRNA levels were normalized to -Actin