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We used the force field parameters for single-protonated phosphorylated Ser (S1P) and Thr (T1P) residues developed by Sticht and co-workers 59

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The mutations were designed based the previous results of the internalization mechanism of IR 12,13

Treatments are vehicle (V), cagrilintide (C, 2 nmol/kg), semaglutide (S, 2 nmol/kg), and calorie-restricted to match the weight of the CS rats (weight matched, WM)

Kynurenine pathway dysfunction in the pathophysiology and treatment of depression: evidences from animal and human studies