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(00:49:40):But other than that, I was actually pretty surprised based off social media, how sick people were, in the bathroom all the time, this and this, and that was a hard thing starting too

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The overnight cultures were used as seed cultures for 1 L of fresh LB medium containing 50 g ml 1 kanamycin and incubated at 37 C until the OD 600 reached 0.8