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Weight loss also typically increases sex hormone-binding globulin (SHBG) levels as insulin sensitivity improves, which affects the interpretation of total versus free testosterone measurements
1 mL Trizol reagent was added to lyse the tissues and extract total RNA
In full-thickness skin wounds, increased VEGF expression and accelerated new blood vessel formation in granulation tissue were demonstrated
A list of candidate biomarkers was determined from the proteins that presented a Welch t-test q- value less than 0.05 (after BenjaminiHochberg correction for multiple testing) and from the top contributors in component 1 and 2 of a sparse partial least square discriminant analysis (sPLS-Da) computed with the Mixomics R package 56
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