The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) ( Recently, we developed a gene transfer-positive cell sorting system using membrane anchoring Twin-Strep-Tag ( Glutathione-S transferase (GST) is a multifunction enzyme that catalyzes the conjugation of glutathione to electrophilic compounds ( Results Design the membrane anchoring glutathione S-transferase fusion maker We use membrane anchoring GST molecule as the affinity tag to allow the transfection-positive cells to be isolated using GSH beads (Figure 1)
Immunocytes can be considered as important target cells of TG, which, if necessary, can produce and deposit TSH and T3, have receptors for these hormones and related genes, which makes them capable of forming new endocrine glands ( Mechanisms underlying the action of TG include both genomic (via nuclear receptors) and non-genomic (membrane) effects
Janet Blanchard Medical Director /Board Certified Plastic Surgeon With over 28 years of experience, Dr
Costa and Labuc, 1994
Relevant markers include malondialdehyde (MDA), 8-isoprostane, reduced glutathione (GSH), and high-sensitivity CRP
