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Experimental paradigm for dual-color retrograde tracing using AAVrg-DIO-EYFP into the DVC region and AAVrg-DIO-tdTomato into the LC region in GLP-1R-ires-Cre mouse
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Additionally, changes in the expression levels of genes that are associated with the significantly altered metabolites and their respective metabolic pathways were studied using RT-qPCR and correlated with alterations in the metabolite levels, followed by correlation to the survival of patients receiving tamoxifen using data obtained from Kaplan Meier webtool
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