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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

Downstream metabolic improvements changes in cortisol, insulin sensitivity, or body composition require longer windows of 612 weeks, and depend substantially on whether DSIP is used as part of a broader supervised metabolic protocol rather than in isolation

(1999) 277:L1138

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