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To determine whether these transcriptomic changes were reflected at the metabolic level, we quantified -hydroxybutyrate, a terminal product of -oxidation, in both cell lysates and culture supernatants

Transient transfection The plasmids were transiently transfected into GBM cells with Lipofectamine 3000 (Invitrogen, CA) according to the manufacturers protocol

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It also helps maintain an acidic environment in the gut, which discourages less friendly bacteria

Bellamine, Nutrients 10 (2018)