For experiments, both flanks of 3-4 weeks male and female mice Braf V600E/+ /Pten fl/fl ( BPT control), Epac1 fl/fl , Epac2 fl/fl , Epac1 +/fl /Epac2 +/fl , and Epac1 fl/fl /Epac2 fl/fl were shaved and 2 L of 5 mM 4HT was applied topically on both flanks for 2 days to generate BPT, Epac1 and Epac2 or Epac1/2 conditional knockout mice referred to hereafter as BPT, Epac1 +/ /Epac2 +/ , Epac1 / /Epac2 / , Epac1 / , and Epac2 / , respectively
The committee is considering an atypical regulatory pathway, allowing these substances to be compounded rather than going through the full, rigorous FDA approval process
References Honda, Yasushi, et al
GSSG is the main source of oxidizing equivalents to provide the adequate environment necessary for favoring disulfide bond formation and proper folding of nascent proteins
Yet animal studies consistently show that oral glutathione can increase tissue levels when properly delivered