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Lysates were cleared twice by centrifugation (12,000g, 10 minutes), and the total protein concentration was measured by Direct Detect Spectrophotometer (Millipore)

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The same chromatographic condition was also used in a study evaluating the effects of oral semaglutide on the pharmacokinetics of lisinopril, warfarin, digoxin, and metformin in healthy subjects [78]

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Open Questions Known risks: clinical, competitive, regulatory, reimbursement, IP, financial, commercial, pricing Assumptions GLP-1 sales for 2023 were approximately $29 billion and it is estimated that 30% of prescriptions could not be filled due to shortages