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The cleared lysate was supplemented with 10 mM imidazole and loaded onto a 5 mL HisTrap column (Cytiva), which was then attached to an Akta Pure M25 instrument (Cytiva), washed using purification buffer supplemented with 20 mM imidazole, and eluted using a linear imidazole gradient from 20 to 300 mM across 12 column volumes

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