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To further investigate whether monotremes evolved a DPP-4 independent pathway to degrade GLP-1, we firstly confirmed the presence of DPP-4 in platypus and echidna sera using a synthetic DPP-4 peptide as a substrate and a DPP-4 inhibitor to prevent this cleavage (Supplementary Fig

However, the half-life of natural GLP-1 in plasma is less than 2 min because it is rapidly enzymatically degraded by DPP-IV

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Swelling, redness, tenderness, and mild bruising are common and generally resolve within one to two weeks