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Accordingly, static calibration involved the determination of the constant signal of the POS recorded at 100% and 0% air saturation (achieved with Na-dithionite, OroboPOS-Service Kit) under the particular experimental conditions (temperature, signal amplification by electronic gain, polarization voltage, stirring speed, medium)

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At the indicated times post-infection, coverslips were washed once with PBS and fixed in 4% paraformaldehyde (Ted Pella Inc., 18505, diluted in 1 x PBS) for 10 min at room temperature