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The body produces GLP-1 in response to all kinds of food intake, but it has a very short duration of action because it is rapidly degraded by DPP-4 enzymes

Discontinuous gradient method: Centrifuge at 2600 g for 1 h

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In these populations, GLP-1 medications may: Suppress appetite in ways that destabilize recovery Mask symptoms without addressing underlying drivers Increase psychological distress related to food, weight, or control Any consideration of GLP-1 use should involve careful medical oversight, psychological evaluation, and ongoing monitoring

Wang YW, Ning D, Peng YZ, Guo YM