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By blocking NNMT, 5-amino-1MQ boosts intracellular NAD+, a coenzyme essential for energy production, DNA repair, and mitochondrial function

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Western blotting Total protein extracts were obtained after cell lysis in 20 mM MOPS (pH 6.5), 1% Triton X-100 (v/w) previously supplemented with 1 mM Na 3 VO 4 , 1 mM NaF, and 1 protease inhibitors (11836170001, Roche)

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