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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)

These derivates from the oxygen play an important role in the microbicide reaction against bacteria and fungi 19,20

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Owodunni OP, Courville EN, Peter-Okaka U, Ricks CB, Schmidt MH, Bowers CA