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This is the first in vivo study to systematically investigate how repeated exposure to SNAC affects gut microbiota, metabolic function, and related health markers

Primary antibodies used included goat anti-choline acetyltransferase (ChAT, 1:1000 dilution, AB144P, Millipore) and rabbit anti-c-Fos (1:1000 dilution, 2250 S, Cell Signaling Technology)

Children under 21 may still qualify under EPSDT

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