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GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

So the lines between 10 and 20 represent 12, 14, 16, and 18 units

During deep sleep, the brain's glymphatic system becomes more active, facilitating the removal of toxins and supporting cellular repair processes

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Ann Thorac Surg 2003;76:1821-7