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In some specific research protocols, alternatives are used: Sterile water for injection (WFI) , when the entire contents of the vial are used at once and a preservative is not needed Physiological saline (0.9% NaCl) , in some applications requiring an isotonic environment Acidic solvents (e.g

Peripherally, pancytopenia is most often observed, but only 1-2 of the major cell types (erythrocytes, leukocytes, platelets) may be depressed in some cases