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healthcare facilities follow this method under the CDCs multi-dose vial protocol

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It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway

Marques, Natlia Nunes, Bruno Paulo Rodrigues Lustosa, Daniel Wagner Castro Lima Santos, Flvio Queiroz-Telles, Sybren de Hoog, Renata R