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NEOs are made up of seven compounds that are classified into three generations: the first (imidacloprid, nitenpyram, acetamiprid, and thiacloprid), the second (thiamethoxam and clothianidin), and the third (dinotefuran) (Li et al

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The ESI source conditions were set as following: sheath gas flow rate of 50 arbitrary units (Arb), auxiliary gas flow rate of 15 Arb, capillary temperature of 320 C, full MS resolution of 60,000, MS/MS resolution of 15,000, collision energy of 10/30/60 in normalized collision energy (NCE) mode, and spray voltage of 3.8 kV (positive) or 3.4 kV (negative)

We fused LacAnc100 to the yeast mating protein Aga2p for surface display and used error-prone PCR to create a library of variants with an average of 17 amino acid changes per gene