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2.8 Proteomic and metabolomic analyses 2.8.1 Sample preparation In postoperative day 7, hippocampal tissues were dissected on ice using sterile instruments ( n = 6 for each groups), rinsed with 0.9% saline, and placed into pre-chilled cryotubes for immediate liquid nitrogen freezing

Methods Antibodies Anti-MBP tag mouse monoclonal antibody and anti c-Jun rabbit monoclonal antibody was used at 1:1000 dilution (Beyotime, AF2912, AF1612), anti-ubiquitin was used at 1:20000 dilution (Abcam, ab134953), anti-GST tag mouse monoclonal antibody was used at 1:5000 dilution (Yeason, 30901ES50), HRP-conjuagated FLAG-tag mouse monoclonal antibody was used at 1:1000 dilution (Yeason, 30502ES60)

PMCID: PMC6597749

Weight loss is more complicated than just eating less and moving more

In peptide research, BPC-157 is frequently studied for its cytoprotective properties and its potential role in supporting tissue repair, recovery responses, and structural healing processes