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Beads were initially equilibrated in 20 mM Tris-HCl pH 8.0, 300 mM NaCl binding buffer, followed by incubation with 50 M His-tagged Syn constructs (Syn WT or Syn 1103 ) for 45 min at 4 C with gentle rotation

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The primary outcome was suicidality, which was defined as a composite of hospital admission for self-harm, suicidal ideation, or completed suicide

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