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Cells were collected by centrifugation (15 min, 4,500 g ) and resuspended in the cold lysis buffer containing 50 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF and 5 mM DTT

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Empirical evidence indicates that vitrified mouse oocytes at the germinal vesicle (GV) and metaphase II (MII) stages exhibit a significant reduction in MMP, leading to mitochondrial dysfunction (47)

Gary Lipman, chairman of the Sunbed Association said: "Nasal tanning sprays have absolutely no place in a professional tanning salon." Read more stories from Cheshire , Lancashire , Greater Manchester and Merseyside on the BBC, watch BBC North West Tonight on BBC iPlayer and follow BBC North West on X .