Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts
However, these comparisons are primarily derived from indirect cross-trial analyses rather than head-to-head randomized trials, thereby limiting the strength of evidence
: 200 500 ,
Where DSIP fits and where it does not Because the evidence is weak, the appropriate role for DSIP in any clinical conversation is narrow
Crowe FL, Key TJ, Allen NE, Appleby PN, Roddam A, Overvad H, Grnbaek H, Tjnneland A, Halkjaer J, Dossus L, Boeing H, Krger J, Trichopoulou A, Dilis V, Trichopoulos D, Boutron-Ruault MC, De Lauzon B, Clavel-Chapelon F, Palli D, Berrino F, Panico S, Tumino R, Sacerdote C, Bueno-de-Mesquita HB, Vrieling A, van Gils CH, Peeters PH, Gram IT, Skeie G, Lund E: The association between diet and serum concentrations of IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 in the European Prospective Investigation into Cancer and Nutrition