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NNMT is an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), producing 1-methylnicotinamide (MNA) and S-adenosylhomocysteine (SAH)

However, for mass spectrometric immunoassays (MSIA), IGF-1 is detected by the measurement of its intrinsic molecular mass and, therefore, alternate forms of IGF-1 that differ in mass can be readily differentiated

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Consequently, targeting these transcription factors represents a promising strategy to eradicate CSCs, inhibit tumor growth, and overcome therapeutic resistance, offering a potential pathway for more effective cancer treatments