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If you're deficient in B12 (common in patients over 50, those with GI conditions, or vegans), oral supplementation won't correct it

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Glutathione plays a crucial role in limiting or preventing the harmful effects induced by free radicals

The F2 progeny was subsequently screened for the desired edit by PCR amplification using the adh-5 forward primer (5-CGATCCAAGTGGCTCCACCGAA-3) and the adh-5 reverse primer (5-TTCCACATCCCAAAAGCGAAACC-3)