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Then, to determine whether the decreased levels of reduced GSH would affect sensitivity to artemisinin treatment, PfMRP1-mutant and PfDd2 parasites were pulse treated with different concentrations of DHA (100 700 nM) for 1 h and the PfMRP1 mutant was significantly more sensitive to DHA treatment compared to PfDd2 wildtype (Supp Fig 3C)