glutathione nhs Scheme 1 Schematic illustration of the reaction pathway involved in AF488 NHS ester | Fluorescence

Assessed were the inferior caval vein (which was occluded), right ovarian vein (which appears as blind vessel due to the infrarenal occlusion), and left ovarian vein (which serves as a bypassing pathway) (Vukojevic et al., 2018)

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Freeze-thaw cycling can disrupt the copper coordination sphere in addition to degrading the peptide backbone, so single-use aliquots are preferable to repeated access of a single stock