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Ive gone back and forth over talking actual numbers, because I know that can be triggering for some people, but Im hoping those people stopped reading in the first few paragraphs, so here goes: right now, I weigh around 250lbs (over 250lbs a few days ago, 248lbs today), or 114kg, and I really think it would make a world of difference to me if I could get to 200lbs or so (89kg)

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In summary, a sample volume containing 500 g of protein was delipidated by adding methanol:chloroform (2:1, v/v) twice